Product Info Summary
SKU: | A04028 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-UBP43 USP18 Antibody
SKU/Catalog Number
A04028
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-UBP43 USP18 Antibody (Catalog # A04028). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-UBP43 USP18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04028)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 25-50 of human UBP43 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04028 is reactive to USP18 in Human
Applications
A04028 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
43.011kDa
Background of USP18
UBP43 (also known as ISG43, Ubl thiolesterase 18, ISG15-specific processing protease, 43 kDa ISG15-specific protease, ubiquitin specific protease 18 or USP18) is a member of the deubiquitinating protease family of enzymes, which removes ubiquitin adducts from a broad range of protein substrates. Deletion of the UBP43 gene in mouse leads to a massive increase of ISG15 conjugates in tissues indicating that UBP43 is a major ISG15-specific protease. UBP43 is the first ISG15-specific protease reported. Both ISG15 and UBP43 genes are known to be strongly induced by interferon, genotoxic stress, and viral infection. Thus UBP43 may be necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
IF Microscopy: User optimized
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of UBP43 expression in HELA nuclear extracts (lane 1), HELA whole cell lysates (lane 2), A431 whole cell lysates (lane 3), JURKAT whole cell lysates (lane 4) and HEK293 whole cell lysates (lane 5). UBP43 at 43KD was detected using rabbit anti-UBP43 Antigen Affinity purified polyclonal antibody (Catalog # A04028) at 1:500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For USP18 (Source: Uniprot.org, NCBI)
Gene Name
USP18
Full Name
Ubl carboxyl-terminal hydrolase 18
Weight
43.011kDa
Superfamily
peptidase C19 family
Alternative Names
EC 3.1.2.15; EC 3.4.19.-; hUBP43; ISG15-specific-processing protease; ISG43; ISG43UBP43; ubiquitin specific peptidase 18; ubiquitin specific protease 18; ubl carboxyl-terminal hydrolase 18; ubl thioesterase 18,43 kDa ISG15-specific protease; Ubl thiolesterase 18; UBP43; USP18 USP18 ISG43, PTORCH2, UBP43 ubiquitin specific peptidase 18 ubl carboxyl-terminal hydrolase 18|43 kDa ISG15-specific protease|ISG15-specific-processing protease|hUBP43|ubiquitin specific protease 18|ubl thioesterase 18|ubl thiolesterase 18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on USP18, check out the USP18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for USP18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UBP43 USP18 Antibody (A04028)
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No publications found for A04028
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-UBP43 USP18 Antibody
Question
Does A04028 anti-UBP43 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
You can see on the product datasheet, A04028 anti-UBP43 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-19
Question
We are currently using anti-UBP43 antibody A04028 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
The anti-UBP43 antibody (A04028) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-20
Question
I have a question about product A04028, anti-UBP43 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Jha
Verified customer
Asked: 2019-06-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04028 anti-UBP43 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-19
Question
I have attached the WB image, lot number and protocol we used for uterus using anti-UBP43 antibody A04028. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-13
Question
I see that the anti-UBP43 antibody A04028 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-12-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-12-25
Question
Is a blocking peptide available for product anti-UBP43 antibody (A04028)?
Verified Customer
Verified customer
Asked: 2018-06-19
Answer
We do provide the blocking peptide for product anti-UBP43 antibody (A04028). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-19
Question
Is this A04028 anti-UBP43 antibody reactive to the isotypes of USP18?
B. Mangal
Verified customer
Asked: 2017-11-06
Answer
The immunogen of A04028 anti-UBP43 antibody is A synthetic peptide corresponding aa 40-49 of human UBP43 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-06